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Introduction Culturing-Aseptic Technique

 

 

Lab 2 Introduction to Culturing & Aseptic Technique “BIO250L” Student Name: Ashleigh Chapman Access Code (located on the lid of your lab kit): AC-SY45LQ1

“Experiment 1- Agar Plate Preparation and Bacterial Inoculation”

“Table 1: Experiment 1 Colony Growth”

“Plate #”

“Condition/Surface” “Growth (color, amount, shape, etc.)”

“1” Control No growth found

“2” Ariborne contamination One small, white round growth

“3” Bathroom doorknob Many very small white dots found

throughout the dish

“4” Trash can lid

1. Large spiderweb like structure in the center

2. 8 yellowish, mostly round structures

3. 15 milky white strucures. Most are a round shape, but

some are oblong

“5” Car gear shifter One large white growth. Appears to be

the consistency of snot

“Post-Lab Questions”

“1. Which plate grew the most bacterial species (based on number of different colonies)? Was this a surprise? Why or why not?”

The inside of my trash can lid grew the most bacterial colonies. There are at least 3 different types. This was not a surprise to me, as this is where we throw out everything from old food to baby diapers. The trash can is also wooden, which may hold more bacteria in. I think that I will be getting rid of that trash can soon.

“2. Was the control plate free of bacterial colonies? If yes, what does this mean for your experiment? If not, what could be the source of the contamination?”

Yes, it was free from contamination. This means that I was able to take the appropriate precautions to keep a clean field to conduct the experiment in .

“3. Did the airborne contamination plate contain any growth? Were any of the colonies similar in appearance to those found on the other plates? How does this plate demonstrate the need for following aseptic technique when culturing bacteria?”

The airborne container had one small growth. It was similar to some of the bacteria found in the trashcan. This shows that you must be careful when culturing bacteria, because it can also become airborne.

“Insert photo of your cultures after incubation with your name clearly visible in the background:”

This study source was downloaded by 100000803078239 from CourseHero.com on 12-17-2021 15:55:55 GMT -06:00

https://www.coursehero.com/file/51126411/Lab-2-worksheetdocx/

 

 

Lab 2 Introduction to Culturing & Aseptic Technique “BIO250L”

This study source was downloaded by 100000803078239 from CourseHero.com on 12-17-2021 15:55:55 GMT -06:00

https://www.coursehero.com/file/51126411/Lab-2-worksheetdocx/

 

 

Lab 2 Introduction to Culturing & Aseptic Technique “BIO250L” “Experiment 2- Bacterial Transfer to a Stab Tube and an Agar Plate”

“Table 2: Initial Reserved Plate Colony Growth Observations”

“Colony ”

“Plate Sample” “Appearance

(color, morphology, etc.)”

“1” Trash lid Orange, round small

“2” Trash Lid Spiderweb like

“3” Trash Lid Yellowish, milky roundish shape. Large

“Table 3: Final Plate and Stab Tube Growth Observations”

“Sample ”

“Form” “Growth?” “Same Appearance as

Initial Plate?” “Effective Transfer?”

“1” “Plate” Small orange dots Yes, only a little smaller yes

“Stab Tube”

nothing No, no growth no

“2”

“Plate” White Spiderweb like

structure Yes, the exact same yes

“Stab Tube”

White spider web like

Similar, appears like it had a harder time

growing inside of the tube

yes

“3” “Plate”

Yellow substance mostly round in nature, along with a spiderweb

like structure

Yes Yes, may have been

contaminated with the web structure also

“Stab Tube”

Yellow dots Yes, but hasFewer

structures yes

“Control ”

“Plate” Contaminated with

large round growth ans small yellow growth

No No, contaminated

“Stab Tube”

No growth No growth yes

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