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Microbial Genetics & Genetic Engineering

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Lab 7 Microbial Genetics & Genetic Engineering BIO250L”

Student Name: Kimberly McAlhany

Access Code (located on the underside of the lid of your lab kit): AC-TI2UVC3

“Pre-Lab Questions”

1. Which DNA nitrogenous bases pair with each other? Which bases are purines, and which

are pyrimidines?

Adenine and guanine are the purines and Cytosine and Thymine are the pyrimidines.

2. How is DNA information used to make proteins? What are the steps of this process?

Enzymes read the information in a DNA molecule and transcribe it into an

intermediary molecule, mRNA. The information contained in the mRNA molecule is

translated for amino acids, which are the building blocks of proteins.

3. Give an example of a scenario in which you would perform PCR vs a scenario in which

you would use recombinant DNA technology.

For PCR, it is when I have an unknown DNA isolated from a specimen in small concentration, and I want to amplify it so it can be identified through gel electrophoresis. For DNA technology it would be to identify the function of a gene.

4. What occurs during each of the three steps involved in the PCR cycle? How has the use

of PCR changed biotechnology?

PCR is based on 3 steps required for any DNA synthesis reaction; 1) denaturation of the template into single strands, 2) annealing of primers to each original strand for new strand synthesis, 3) extension of the new DNA strands from the primers.

5. How could you take a protein with a known sequence of amino acids and use it to

create an artificial gene?

With help from known protein sequences, you can make its most probable nucleotide sequence based on the frequency of tRNA that is present in the organism in which we need to express the proteins. We will then artificially make the sequence and amplify it by using PCR.

This study source was downloaded by 100000792411801 from CourseHero.com on 11-13-2021 20:30:17 GMT -06:00

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What factors do you feel influenced the nurse theorist’s definitions and descriptions of the components of the metaparadigm in their respective models?

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What are the tenets of the metaparadigm as defined by Nightingale, Johnson, Orem, Roy, Leininger, Neuman, Rogers, and Watson? How does each theorist differ from the others? Please discuss these theorists only.

·         What factors do you feel influenced the nurse theorist’s definitions and descriptions of the components of the metaparadigm in their respective models? Explain.

·         What are four salient contributions of nursing theorists to the development of nursing science? Why did you choose these particular contributions?

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Suppose that you have a house guest from another country.  He is curious about the lifestyles that we tend to lead in the United States.  He wants to know why there seems to be such a big problem with nutrition and lack of exercise.  Explain to him why nutrition and exercise are important to the health of our society as a whole

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Your assignment is to write a 2-3 page essay in which you address the scenario below.
Suppose that you have a house guest from another country.  He is curious about the lifestyles that we tend to lead in the United States.  He wants to know why there seems to be such a big problem with nutrition and lack of exercise.  Explain to him why nutrition and exercise are important to the health of our society as a whole.
Case assignment expectations:
Use information from the modular background readings as well as any good quality resource you can find. Please cite all sources and provide a reference list at the end of your paper.
LENGTH: 2-3 pages typed and double-spaced.
The following items will be assessed in particular:
1. Your ability to explain why lifestyle factors such as nutrition and exercise are important to the health of a society
2. Some in-text references to modular background readings (APA formatting preferred)
Be sure that your opinion is justified with evidence from the literature. Try to use several scholarly references for each assignment. All references should be cited properly in the text of the paper as well as at the end.  Please limit your response to 3 pages maximum.  Assignments are due on the Monday after a module ends.

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What is the meaning and significance of death in light of the Christian narrative?

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Write a 750-1,000 word paper analyzing Woterstorff’s reflctions in Lament For a Son. In addition, address Kubler-Ross’ five stages of grief, as they are expressed throughout Lament for a Son, and respond to the following questions:

How does Wolterstorff find joy after his loss? What is the meaning and significance of death in light of the Christian narrative? How does the hope of the resurrection play a role in comforting Wolterstorff? Include three sources including the textbooks, bible and other reliable/academic sources.

Prepare this assignment according to the APA guidelines found in the APA Style Guide, located in the Student Success Center. An abstract is not required.

This assignment uses a rubric. Please review the rubric prior to beginning the assignment to become familiar with the expectations for successful completion.

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Future Considerations for the APRN Role

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Future Considerations for the APN Role

 

Discussion Prompt

There are three troublesome issues that have emerged in the advancement of APRN practice. These issues in APRNs include 1) the clear role development and morphed to fill gaps in services; 2) the lack of clarity regarding role uniqueness, and 3) the dearth in nursing-specific outcomes. From your readings, this week, discuss 1 of the 3 questions. Begin your discussion with the question you will be addressing.

1. Why might the development and morphing of roles based on gaps in medicine be problematic for the advancement of APRNs? What actions might a nurse take to reduce this?

2. In what ways can an APRN distinguish his or her role from other nursing roles and medical counterparts? Why might this be important?

3. How can APRNs encourage nursing-based outcome studies?

 

Expectations

Initial Post:

APA format with intext citations

Word count minimum of 250

References: 2 high-level scholarly references within the last 5 years in APA format.

Plagiarism free.

Turnitin receipt.

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Which choices below best reflect the problem statement for the instructor-assigned article?

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  1. Which choices below best reflect the problem statement for the instructor-assigned article?
    1. In an effort to address the shortages of nurses with undergraduate and graduate nursing degrees, nursing programs have been measuring student retention, attrition, and graduation rates.
    2. There is a paucity of research regarding the relationship of emotional intelligence, psychological empowerment, resilience, spiritual well-being, and academic success in the context of nursing students.
    3. The total score of the psychological empowerment scale represents psychological empowerment at that moment in time. This will not necessarily be the same when measured at a different point in time.
    4. Nurse educators need to examine ways to promote student success by improving our current methodologies and practices.

 

  1. Which of the choices below best reflects the purpose statement for the instructor assigned article?
  1. Graduate prepared nurses will be needed to replace the large number of retiring faculty in Canada and the United States. In addition, nurse practitioners are needed to help alleviate the family physician shortage.
  2. The current rise in employment is improving forecasts for the future supply of registered nurses; however sizable shortages are still projected for the following decade in the United States.
  3. Many universities and admission departments dedicate substantial time and money for the recruitment and admission of nursing students.
  4. The purpose of the correlational study was to describe the relationship between emotional intelligence, psychological empowerment, resilience, spiritual well-being, and academic success and undergraduate and graduate nursing students.

 

  1. According to Grove, Gray, and Burns (2015), which of the following statements are important when considering the significance and relevance of a study’s problem and purpose? (Select all that apply.)
    1. Does it consider ethical principles in its design?
    2. Does it predict the non-significant findings anticipated in the study?
    3. Does it influence nursing practice?
    4. Does it identify the population to whom they intend to generalize?
    5. Does it promote theory testing or development?
    6. Does it identify extraneous variables?
    7. When considering the feasibility of a study’s problem and purpose, Grove, Gray, and Burns (2015) suggest that several areas should be evaluated, including: researcher expertise, money commitment, ethical considerations, and availability of subjects, facilities, and equipment. Which of the following statements accurately assesses the feasibility of this article?
  2. Funding sources for the study were identified in the article.
  3. The author’s credentials to design and conduct research are fully described.
  4. 100% of the eligible subjects contacted participated in the study.
  5. Evidence of protection of the subjects’ rights was mentioned in this article.

 

Questions 5 – 7: Review of the Literature.  (For help with these questions, refer to chapters 6 & 12.)

  1. According to Grove, Gray, and Burns (2015), which one of the following is NOT a major purpose of the review of literature (ROL):
  2. Describing the current knowledge of the practice problem
  3. Identifying gaps in the knowledge base of the practice problem
  4. Explaining how the current study contributes to the knowledge being built
  5. To explain the reasons behind the selection of the statistics used in the study.
  6. Select three MAJOR topics covered in the review of literature (ROL) from the list below:
    1. A descriptive correlational design was used. The study was conducted in a private Catholic University.
  7. The current rise in employment is improving forecasts for the future supply of registered nurses; however sizable shortages are still projected for the following decade in the United States.
  8. The purpose of this study was to describe the relationship between emotional intelligence, psychological empowerment, resilience, spiritual well-being, and academic success in undergraduate and graduate nursing students.
  9. In an effort to address the shortages of nurses with undergraduate and graduate nursing degrees, nursing programs have been measuring student retention, attrition, and graduation rates.
  10. The nursing profession should be concerned about attrition rates for masters and doctoral nursing students as well. Attrition rates for masters programs range from 10 to 75%.
  11. f. There is a paucity of research regarding the relationship of emotional intelligence, psychological empowerment, resilience, spiritual well-being, and academic success in the context of nursing students.

 

  1. Current knowledge in the review of literature (ROL) (all information included before the “Methods”) is considered to be articles that are within 5 years of the publication date of the article. This is often assessed by reviewing the citations that are used in the ROL and counting the number that meet this criteria.   Which number below most closely reflects the number of current citations in the ROL? (HINT-start with the year 2014 as the year published in the journal.)

 

  1. 10
  2. 15
  3. 20
  4. 25

Questions 8 – 10: Study Framework. (For help with these questions, refer to chapters 7 & 12)

  1. Which of these statements best describes this study’s research framework?
  2. This study has an implicit framework, which is not fully developed.
  3. The conceptual framework supporting the research is underpinned by social exchange theory and Bandura’s theory of self-efficacy.
  4. The authors developed the study’s conceptual framework based on Watson’s Theory of Caring to describe the concept of caring for the self as a nursing student.

 

  1. What are some of the key concepts in this study’s conceptual framework?
  2. This study does not have any clearly defined concepts within a framework because it has an implicit framework.
  3. Nursing student depression is defined as a psychosocial phenomenon occurring within 12 months of starting in either an undergraduate or graduate program.
  4. The authors clearly identify the concepts of the framework in a map or model.

 

  1. Which one of the statements below is an example of a relational statement from the conceptual framework?
  2. Feelings of psychological empowerment will have an effect on student depression levels
  3. This study does not have any clearly defined relational statements because it has an implicit framework.
  4. Researchers found that managing emotions was positively correlated with academic success.

 

 

Questions 11 – 15:  Research objectives, questions, or hypotheses and research variables. (For help with these questions, refer to chapters 5 & 12)

 

  1. The authors state that the research objective, question, or hypothesis was “describe the relationship between emotional intelligence, psychological empowerment, resilience, spiritual well-being, and academic success in undergraduate and graduate nursing students” on page 918. This is best described as a
  2. Research objective
  3. Research question
  4. Research hypothesis
  5. None of the above
  6. Which of these would be considered a MAJOR study variable (select all that apply)?
  7. emotional intelligence
  8. psychological empowerment
  9. undergraduate and graduate retention rates
  10. costs of student recruitment
  11. resilience
  12. spiritual-well being
  13. What is the conceptual definition (as defined in the review of the literature) of the following study variable: resilience
  14. The conceptual definition of resilience is not clearly provided in the review of the literature.
  15. The factors that influence nursing academic success need to be better understood.
  16. After students are admitted, they are to be afforded resources that will foster their persistence in the nursing program as well as promote their academic success.
  17. Admissions staff are tasked with the ever more difficult charge of distinguishing applicants who can be successful.

 

  1. What is the operational definition (as defined in the methods section) of the following study variable: resilience.
  2. Recruiting qualified applicants is just the beginning step in fostering program completion.
  3. Mayer-Salovey-Caruso Emotional Intelligence Test (MSCEIT)
  4. The factors that influence nursing academic success need to be better understood.
  5. The Wagnild and Young Resilience Scale (RS).
  6. Which demographic variables were assessed by the author for this study? (Select all that apply.)
  7. Age
  8. Education level
  9. Years of nursing experience
  10. Marital status
  11. Admission cut-off criteria
  12. Race

 

Questions 16- 19:  Research Design (For help with these questions, refer to chapters 1, 8, & 12)

  1. Which phrase best describes the research design of this study? (Select the three that apply.)
  2. Descriptive
  3. Correlational
  4. Nonexperimental
  5. Quasi-experimental
  6. Experimental
  7. Mixed methods

 

  1. Which phrase best describes the time element of the research design of this study?
  2. a. Cross-sectional design
  3. Longitudinal design
  4. None of the above

 

  1. Does the study include a treatment or intervention described in the methods section?
  2. The Spiritual Well-Being Scale (SWBS) used in the study may be considered a treatment or intervention.
  3. 516 students were invited to participate in the intervention.
  4. A control group of accounting students were also asked to complete the survey.
  5. This study was not designed with a treatment or intervention.

 

  1. Does the author specifically mention that a pilot study was done prior to conducting this study?  (Hint: look at the words “prior to this study”.)
  2. Yes
  3. No

 

  1. The authors indicate on page 920 of the article that the institutional review board (IRB) authorization was acquired. This indicates than an IRB gave approval to conduct the research. In addition, anonymity and confidentiality was protected.  Per Grove, Gray, and Burns (2015), which of the following would NOT be considered essential information for informed consent?  (Select all that apply.)

 

  1. a statement of the research purpose and any long-term goals of the study
  2. a copy of the abstract of the article that will be used in the publishing journal.
  3. an explanation of the procedures to be followed in the study
  4. a complete list of references to be used in the study.

 

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Identify a Public Health Concern that contributes to health risks or disease.

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Identify a Public Health Concern that contributes to health risks or disease.

Public Service Announcement

Identify a public health concern that contributes to health risks or disease. As a governmental official, prepare a public service announcement (PSA) in the form of a two-page brochure to educate the public on this concern. Remember to address the following:

  1. A brief explanation of the concern, including a definition
  2. How the concern impacts public health, with current statistics
  3. What citizens can do to protect themselves
  4. How your agency can address the concern, including measures and surveillance
  5. Frequently Asked Questions (FAQs) –  with specific items
  6. Whom to contact for additional details (e.g., WHO, Ministry of Health), including an address and phone number.

Your brochure should meet the following structural requirements:

– Two-page brochure that includes all elements detailed above

– Conforms APA writing standards; be sure to cite any statistics or other information, as appropriate.

– Add cited image

– References pages could be included on a separate page or the back, to not draw away from the brochure.( not older than 5 years back)

– In MS Word there is an option for creating a brochure, you can use images, bulleted, lists, etc, to be creative.

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  1. .Identify a Public Health Concern that contributes to health risks or disease.

Identify a Public Health Concern that contributes to health risks or disease.

 

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environmentally friendly fabrics

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urgentfast

In task 2-3, you will submit a short paper of 2–3 pages. Determine your target market category and how and why these consumers embrace fashions that are made of environmentally friendly fabrics. You should research and define your product’s targeted customers and describe their characteristics.

Description of a Target Market: Occupation, gender ratio, average age, geographics/location, psychological shopping habits, demographic, and lifestyle preferences. Gauge the importance of sustainable products to this customer. For example, if you are creating for kids’ wear, indicate age(s) of child; however, you will need to think of a profile for the primary caretaker of the child (parent, grandparent, guardian) so that you can market to the caretaker properly.

By Module Two, you should have an idea of the market category you are choosing for this garment line, and you may choose ONE of the following: women’s, men’s, juniors, petite women, plus-sized women, or kids’ wear. Indicate the subcategory/style description, for example, sportswear (everyday wear), activewear (such as yoga wear), playwear, or sleepwear. Remember, you will be using sustainable fabrics, so keep in mind which target market would benefit best from eco-friendly garments.

Keep in mind the size range of this collection. Choose the appropriate size range for your market category. For example, if you are doing kids’ wear, you may choose to target toddlers (size range is 2T-3T-4T). An example of junior girls’ sizing range is 0–15.

The format should be a short paper. This milestone is graded for the quality of the research/data you uncover and validate (cite all information), compilation of the information, the content of the descriptions of each the features of your specified target customer, and the way the data is expressed as it applies to this project.

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Antimicrobial agents essential in the treatment of various bacterial infections

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Antimicrobial agents essential in the treatment of various bacterial infections

Assignment: Antimicrobial Agents

Antimicrobial agents are essential components in the treatment of various bacterial infections, fungi, and protozoans. Prior to the discovery of antimicrobial agents, treatment options for patients with bacterial infections were limited. For many patients, treatment often resulted in the amputation of limbs or even death. Today, treatment options for bacterial infections typically have a more positive prognosis. Due to the various types of infections presented in patients, it is essential to be able to identify the underlying cause of the infection—whether bacterial or viral—before recommending drug treatments. This will help you identify whether or not an antimicrobial agent would be appropriate and which specific agent would target the infection. In this Assignment, you consider the appropriate use of antimicrobial agents for infections.

To prepare:

  • Review this week’s media presentation on principles of antimicrobial therapy, as well as Chapter 8 of the Arcangelo and Peterson text.
  • Consider the categories of antimicrobial agents.
  • Think about the differences between viral and bacterial infections.
  • Reflect on why proper identification of the infection is key to selecting the proper antimicrobial agent.

By Day 7

Write a 2- to 3- page paper that addresses the following:

  • Describe the categories of antimicrobial agents.
  • Describe differences between viral and bacterial infections.
  • Explain why proper identification of viral and bacterial infections is key to selecting the proper antimicrobial agent.

Assignment: Antimicrobial Agent

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Antimicrobial agents essential in the treatment of various bacterial infections

Antimicrobial agents essential in the treatment of various bacterial infections

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Discuss why the current nursing practice needs to be changed.

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TRANSLATIONAL RESEARCH FOR PRACTICE & POPULATIONS

Competency 7000.1.1: Evaluating Comprehensive Literature Reviews – The graduate evaluates comprehensive literature reviews for historical context, completeness, applicability, reliability, organization, and quality of information sources.
Competency 7000.1.7: Translation of Research in Evidence-Based Practice – The graduate interprets evidence and evaluates its applicability to nursing practice.

Introduction:

One of the key roles of the master’s-prepared nurse is to lead practice change across the full spectrum of healthcare settings. Master’s-prepared nurses must be able to translate the existing body of knowledge around a phenomenon of interest (accessible through published research studies) to inform decision making related to nursing practice.

Requirements:

  1. Identify a current nursing practice within your healthcare setting that requires change.
  2. Describe the current nursing practice.
  3. Discuss why the current nursing practice needs to be changed.
  4. Identify the key stakeholders within your healthcare setting who are part of the current nursing practice.
  5. Describe the role eachidentified key stakeholder will play to support the proposed practice change.
  6. Create an evidence critique table (see “Sample Evidence Critique Table”).
  7. Identify five sources from scholarly peer-reviewed journals, which adhere to the following standards:
  8. Each source must be published within the past five years.
  9. Each source must relate to the change identified in part A.
  10. Each source must be listed in the table using APA format.
  11. Identify the evidence strength of each chosen source, using the Strength of Evidence information in the study plan.
  12. Identify the evidence hierarchy of each chosen source, using the Evidence Hierarchy information in the study plan.
  13. Develop an evidence summary based on the findings from part C that includes eachof the five sources used (suggested length of 1–2 pages for all sources).
  14. Recommend a specific best practice based on the evidence summary developed in part D.
  15. Identify a practice change model that is appropriate to apply to the proposed practice change.
  16. Justify why you chose the practice change model.
  17. Explain how to apply the identified model to guide the implementation of the proposed practice change.
  18. Discuss possible barriers to successful implementation of the proposed practice change.
  19. Discuss any possible ethical implications that may arise while planning or implementing the proposed practice change.
  20. When you use sources, include all in-text citations and references in APA format.

Note: For definitions of terms commonly used in the rubric, see the Rubric Terms web link included in the Evaluation Procedures section.

Note: When using sources to support ideas and elements in an assessment, the submission MUST include APA formatted in-text citations with a corresponding reference list for any direct quotes or paraphrasing. It is not necessary to list sources that were consulted if they have not been quoted or paraphrased in the text of the assessment.

Note: No more than a combined total of 30% of a submission can be directly quoted or closely paraphrased from outside sources, even if cited correctly. For tips on using APA style, please refer to the APA Handout web link included in the APA Guidelines section.

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